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WP 7.1: Role target organ toxicity (brain)

 

Articles

M. Aschner, E.D. Levin, C. Suñol, J.O. Olopade, K.J. Helmcke, D.S. Avila, D. Sledge, R.H. Ali, L. Upchurch, S. Donerly, E. Linney, A. Forsby, P. Ponnoru, and J.R. Connor. Gene-Environment Interactions: Neurodegeneration in Non-Mammals and Mammals. Submitted

 

Gustafsson H., Runesson J., Lundqvist J., Lindegren H., and Forsby A. The use of the human neuroblastoma SH-SY5Y cell line for estimation of acute systemic toxicity. Submitted.

Galofré M., Babot Z., García D.A., Iraola S., Rodrìguez-Farré E., Forsby A. and Suñol C. (2009). GABAA receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity. Neurotoxicology and Teratology (in press, doi:10.1016/j.ntt.2009.01.010).

 

Folch J., Alvira D., López-Querol M., tajes M., Sureda F.X., Forsby A., Rimbau V., Camins A. and Pallas M. evaluation of transcriptional activity of caspase 3 gene as a marker of acute neurotoxicity in rat cerebellar granual cells. Toxicology In Vitro (In Press, doi:10.1016/j.tiv.2009.09.023)

 

Folch J, Yeste-Velasco M, Alvira D, de la Torre AV, Bordas M, López M, Sureda
FX, Rimbau V, Camins A, Pallàs M. Evaluation of pathways involved in
pentachlorophenol-induced apoptosis in rat neurons. Neurotoxicology. 2009
May;30(3):451-8. Epub 2009 Feb 10. PubMed PMID: 19442831.

 

Forsby A., Bal-Price A., Camins A., Coecke S., Fabre, N., Gustafsson H., Honegger P., Kinsner-Ovaskainen A., Pallas M., Rimbau V., Rodríguez-Farré E.,  Suñol C., Vericat J.A. and  Zurich, M.G. Neuronal in vitro models for the estimation of acute systemic toxicity. Toxicology In Vitro (In Press, doi:10.1016/j.tiv.2009.07.017)

 

Folch, Yeste-Velasco,  Alvira , Sureda, Rimbau, Bordas, López, Camins and Pallàs. Evaluation of pathways involved in pentachlorophenol-induced apoptosis in rat neurons. Neurotoxicology 2009 (accepted).

 

Yeste-Velasco,  Alvira , Sureda, Rimbau, Forsby, Pallàs, Camins andFolch.  cDNA low density array analysis of colchicine neurotoxicity in rat cerebellar granular neurons. Neurotoxicology. 29(2):309-17, 2008.

 

Suñol C., Babot Z., Fonfría E., Galofré M., García D., Herrera N., Iraola S. and Vendrell I.  Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity”. Toxicology In Vitro. 22 (5), 1350-5. 2008.

 

Bal-Price A.K., Suñol C., Weiss D.G., van Vliet E. and Remco H.S. Application of in vitro neurotoxicity testing for regulatory purposes: Symposium III Summary and Research Needs. Neurotoxicology. 29(3), 520-31. 2008.

 

Forsby A., Camins A., Coecke S., Fabre, N., Gustafsson H., Honegger P., Kinsner-Ovaskainen A., Pallas M., Price A., Rimbau V., Rodríguez-Farré E.,  Suñol C., Vericat J.A. and  Zurich, M.G. Neuronal in vitro models for the estimation of acute systemic toxicity. Toxicology In Vitro, 2008 (submitted).

 

Galofré M., Babot Z., García D.,  Iraola S., Rodriguez-Farré E., Forsby A. and Suñol C. GABAA receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity. Neurotoxicology and Teratology, 2008  (under revision).

 

Bal-Price A.K., Suñol C., Weiss D.G., van Vliet E. and Remco H.S.  Application of in vitro neurotoxicity testing for regulatory purposes: Symposium III Summary and Research Needs. Neurotoxicology (submitted).  Manuscript.


Forsby A. and Blauuboer B.  Integration of In Vitro Neurotoxicity Data with Biokinetic Modeling for the Estimation of In Vivo Neurotoxicity. Human and experimental toxicology, 26; 333-338, 2007. 

 

Folch J., Yeste-Velasco M.,  Alvira D., Vázquez de la Torre A., Bordas M., López M., Sureda F.X., Rimbau V., Camins A. and Pallàs M. Evaluation of pathways involved in pentachlorophenol-induced apoptosis in rat neurons.  Submitted to Neurotoxicology. Manuscript 2008.


Honegger P., Tavel D. and Zurich M.G. Aggregating brain cell cultures as a model for acute neurotoxicity testing. Neurotoxicoloy (in press).  Manuscript.


Suñol C., Babot Z., Fonfría E., Galofré M., García D., Herrera N., Iraola S. and Vendrell I.  Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity. Toxicology In Vitro (submitted).  Manuscript.


Yeste-Velasco M, Alvira D, Sureda F.X., Rimbau V, Forsby A., Pallàs M., Camins A. and Folch J. cDNA low density array analysis of colchicine neurotoxicity in rat cerebrellar granular cell. Neurotoxicoloy (in press).  Manuscript.

 

Abstracts

Suñol C. and Forsby A. (2009). Combination of cell based assays determining GABAA receptor activity and Cell Membrane Potential for in vitro testing of neurotoxicity. Forinvitox event May 12-14. Stockholm. Oral presentation.

Cecilia Clemedson, Thomas Cole.2, Richard Clothier3, Sandra Coecke4, José Enrique O´Connor5, Bas Blaauboer6, Maria José Gómez-Lechón7, Joan Albert Vericat8, Anna Forsby 9, Michael P. Ryan10, José Castell7, Agnieszka Kinsner4 and Pilar Prieto4 (2009). Overview of the ACuteTox project and multivariate analysis of the in vitro data. WC7 in Rome 29 August-3 September

Forsby A., Camins A., Coecke S., Fabre, N. , Gustafsson H., Honegger P., Kinsner-Ovaskainen A., Pallas M., Price A., Rimbau V., Rodríguez-Farré E.,  Suñol C., Vericat J.A.and  Zurich, M.G.   Neuronal in vitro models for the estimation of acute systemic toxicity. The 15th International Congress on In Vitro Toxicology, 25 to 28 Sept 2008, Stockholm. Oral presentation.

 

Lundqvist J., Runesson J., Helena Gustafsson H. and Anna Forsby. (P16).The influence of exposure time on the inhibitory effect of the acetylcholine esterase activity in human neuroblastoma SH-SY5Y cells. The 15th International Congress on In Vitro Toxicology, 25 to 28 Sept 2008, Stockholm, Sweden. Poster presentation 

 

Galofré M., Babot Z., García D.A., Iraola S., Herrera N. and Suñol C. GABAA receptor and cell membrane potential as functional endpoints in cultured neurons to evaluate chemicals for human acute toxicity. 45th Congress of the European Societies of Toxicology (EUROTOX 2008). October 2008, Rodes, Greece.

 

Folch J., Rimbau V., Alvira D., Sureda F.X., Camins A. and Pallàs M. Caspase-3 gene: sensor of acute neurotoxicity. The XXX Congreso de la Sociedad Española de Farmacologia, september 2008, Bilbao. Spain. Poster presentation

 

Babot Z., Galofré M., García D., Herrera N., Iraola S., Sabater D. and Suñol C. The GABAA and NMDA-glutamate receptors as targets of toxicity. XXVIII Meeting of the Spanish Group on Neurotransmission (GEN), Aranjuez, Spain, 12-14 December 2007. Oral presentation.


Forsby A. An in vitro test strategy for prediction of acute neurotoxicity. 23rd SSCT workshop in collaboration with the Estonian Toxicology Society, Toila, Estonia 2005.  Oral presentation.


Forsby A. Integration of In Vitro Neurotoxicity Data with Biokinetic Modeling for the Estimation of In Vivo Neurotoxicity. 10th workshop of the International Neurotoxicology Association, Poorvo, Finland 2005. Oral presentation. Image Abstract pdf 15 kB


Forsby A., Gustafsson H., Honegger P. and Suñol C. RECEPTOR-MEDIATED ACUTE SYSTEMIC TOXICITY STUDIED IN NEURONAL CELL MODELS. 24th SSCT Workshop on in vitro toxicology, September 7-9th, Grinda, Stockholm 2006.  Oral presentation.

 

Forsby A. Neuronal in vitro models for the estimation of acute systemic toxicity. 15th International Congress on In Vitro Toxicology, 25 - 28 Sept 2008, Stockholm, Sweden.  Oral presentation.  Image Abstract pdf 15 kB


Galofré M., García D., Babot Z., Iraola S., Collazo O., Herrera N. and Suñol C. Neuronal endpoints in primary neuronal cultures to evaluate and predict human acute neurotoxicity. XIX meeting of the Spanish Society for Neuroscience, Valencia, Spain, 5-7 September 2007. Poster.  Image Abstract pdf 16 kB


Gustafsson H., Runesson J. and Forsby A. Acetylcholine esterase activity as an endpoint for prediction of acute human neurotoxicity. 23rd SSCT workshop in collaboration with the Estonian Toxicology Society, Toila, Estonia 2005.  Oral presentation.


Gustafsson H., Runesson J. and Forsby A. Prediction of human acute neurotoxicity – development of a test strategy in vitro. 23rd SSCT workshop in collaboration with the Estonian Toxicology Society, Toila, Estonia 2005.  Oral presentation.


Gustafsson H. and Forsby A. CELL MEMBRANE POTENTIAL -A PROMISING ENDPOINT FOR THE PREDICTION OF ACUTE HUMAN NEUROTOXICITY. 24th SSCT Workshop on in vitro toxicology, September 7-9th, Grinda, Stockholm 2006.  Oral presentation.


Gustafsson H, Runesson J, Lindegren H,  Axelsson V and Forsby A. THE USE OF THE HUMAN NEUROBLASTOMA SH-SY5Y CELL LINE FOR ESTIMATION OF ACUTE SYSTEMIC TOXICITY.  25th SSCT Workshop on in vitro toxicology, 19-22 September Salzau, Germany 2007. Oral presentation. Image Abstract pdf 18 kb



Pallàs M. Drug-induced neurotoxicity regulates apoptosis, oxidative stress and cell cycle control gene expression. XXIX Meeting of the Spanish Society for Pharmacology, Alcalá de Henares, Spain, September 2007. Poster. Image Abstract pdf 62 kB


Rodríguez-Farré E., Pertusa M., García-Matas S., Suñol C., Sanfeliu C. and Cristòfol R. Astrocytes aged in vitro display an impaired neuroprotective capacity in cortical neurons: a tool in screening for neurotoxicity. 11th Meeting of the International Neurotoxicology Association, Asilomar Conference Center, Pacific Grove, California, USA, 10 – 15 June 2007. Poster. Image Abstract pdf 78 kB


Suñol C., García D.A. Iraola S. and Babot Z. Assessment of neurochemical end-points in primary neuronal cultures to predict acute toxicity of neurotoxic compounds. VI Symposium of Experimental Neurobiology. Catalan Society of Biology. Barcelona, Spain. 22 – 23 November 2006.   Poster. Image Abstract pdf 17 kB


Suñol C. In vitro neural models to evaluate human acute neurotoxicity. The European project ACUTETOX. 11th Meeting of the International Neurotoxicology Association, Asilomar Conference Center, Pacific Grove, California, USA, 10 – 15 June 2007. Oral presentation. Image Abstract pdf 15 kB


Suñol C. Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity. 25th SSCT Workshop on in vitro toxicology, 19-22 September Salzau, Germany 2007. Oral presentation. Image Abstract pdf 20 kB


Yeste M, Alvira D., Sureda F.X.,  Folch J.,  Crespo-Biel N., Rimbau V., Camins A. and  Pallàs M. EVALUATION OF NEUROTOXICITY IN CEREBELLAR GRANULE CELLS An In Vitro approach to In Vivo Toxicity. Simposi de la Societat Catalana de Neurobiologia held in Barcelona, December 2006.   Poster.

 

 

Others

Hörnblad L. and Runesson J. Establishing a method to determine neurospecific toxicity by measuring the AchE-activity in human neuroblastoma SH-SY5Y cell. D-course project, Neurochemistry and neurotoxicology, Stockholm university, 2005. Student report.


Mukuru A. and Stjärnmark-Hirschberg L. Determining noradrenalin uptake in human neuroblastoma cells (SH-SY5Y) after exposure to amphetamine and caffeine. Project Report, D-course in Neurochemistry (KE4820), Stockholm university, 2006. Student report.


Runesson J. A toxicity study measuring the AChE Activity in human  neuroblastoma SH-SY5Y cells. Project Report, Stockholm university, 2005. Student report.